講師資料
Talks：
Transcriptional repression by Cryptochrome in the absence of Period
Name：
邱奕穎(Yi-Ying Chiou)
Position：
Assistant Professor
Affiliation：
Graduate Institute of Biochemistry, National Chung Hsing University, Taichung City, Taiwan
Email：
Photo：
Research Interests：
Molecular mechanisms of the mammalian circadian clock
Regulation and functional characterization of Photolyase/Cryptochrome families
Transactional regulation with other biological events (DNA damage, Repair, Circadian Rhythm)
Selected Publications：
1. YI-YING CHIOU, Tzu-Ying Li, Yanyan Yang, Aziz Sancar (2020, Dec). A sextuple knockout cell line system to study the differential roles of CRY, PER, and NR1D in the transcription-translation feedback loop of the circadian clock. Frontiers in Neuroscience, 14;14:616802
2. Wang X, Jing C, Selby CP, Chiou YY, Yang Y, Wu W, Sancar A, Wang J. Comparative properties and functions of type 2 and type 4 pigeon cryptochromes. Cell Mol Life Sci. 2018 Sep 27. doi: 10.1007/s00018-018-2920-y
3. Yanyan Yang, Ogun Adebali, Gang Wub, Christopher P. Selbya, Yi-Ying Chiou, Naim Rashid, Jinchuan Hu, John B. Hogenesch, and Aziz Sancar. Cisplatin-DNA adduct repair of transcribed genes is controlled by two circadian programs in mouse tissues. PNAS 2018 115(21), E4777-E4785
4. Yi-Ying Chiou, Jinchuan Hu , Aziz Sancar , and Christopher P. Selby. RNA polymerase II is released from the DNA template during transcription-coupled repair in mammalian cells. Journal of Biological Chemistry 2018 293 (7), 2476-2486
5. Wentao Li, Jinchuan Hu, Ogun Adebali, Sheera Adara, Yanyan Yang, Yi-Ying Chiou, and Aziz Sancar Human genome-wide repair map of DNA damage caused by the cigarette smoke carcinogen benzo[a]pyrene. PNAS 2017 114(26), 6752-6757
6. Ogun Adebali, Yi-Ying Chiou, Jinchuan Hu, Aziz Sancar, Christopher P. Selby Genome-wide transcription-coupled repair in Escherichia coli is mediated by the Mfd protein. PNAS 2017 114(11), E2116-E2125
7. Yi-Ying Chiou*, Yanyan Yang*, Naim Rashid*, Rui Ye, Christopher P. Selby, Aziz Sancar (* equal contribution) Mammalian Period represses and de-represses transcription by displacing CLOCK-BMAL1 from promoters in a Cryptochrome-dependent manner. PNAS 2016 113 (41), E6072-E6079
Abstract：
Circadian rhythm is a physiological mechanism with daily periodicity to adapt to the daily environmental changes. Regulation of circadian rhythm affect behavior (sleep-wake cycle) and other physiological functions. The molecular mechanism of circadian regulation is through the transcription-translation feedback loop. Cryptochrome protein (CRY) and Period protein (PER) play a negative feedback function in the mammalian system. CRY and PER could interact with each other. Thus, they were considered to regulate circadian rhythm as a heterodimer. However, the ratio of CRY and PER proteins are different at different times of the day, suggesting the existence of their independent functions. This talk will focus on introducing the mammalian transcription-translation feedback loop and discuss the independent function of CRY in transcriptional repression based on the research results from in celluo biochemical system in the lab.